Yehudit Naot was born in Israel in 1944. A Professor in the Technion Medical Department and a scientist, she held a D.Sc. in Biology and a B.Sc. in Chemistry from the Technion Israel Institute of Technology.
Naot was a member of the Shinui party since 1975, and was active in its council and secretariat. She served as a member of the Haifa Municipal Council (1978-1979, 1983-1987 and 1989-1997) and as Deputy Mayor of Haifa (1989-1991 and 1993-1996).
She was a Member of Knesset since 1999 serving on the Internal Affairs and Environment, Education and Culture Committees. She was also a member of the Parliamentary Inquiry Committee on the Continuing Financial Crisis of the Local Governments and the Parliamentary Inquiry Committee on the Trading of Women.
In February 2003, Yehudit Naot was appointed Minister of the Environment. She resigned this post in October 2004 due to ill health and passed away in December 2004.