Ami Ayalon was born in Tiberias, Israel in 1945. He received a B.A. in Economics and Political Science from Bar Ilan University in 1980 and an M.A. in Public Administration from Harvard University in 1992.
Ayalon served his entire military service in the Israel Navy, from his enlistment in 1963, volunteering for the navy commando unit. In 1969, Ayalon was decorated with the Medal of Valor, Israel's highest award. In 1979, Ayalon was appointed commander of the navy commando. Receiving the rank of admiral, he served as commander of the Israel Navy from 1992-1996.
From 1996-2000 Ayalon served as Director of the Israel Security Agency, and from 2001-2005 as Chairman of the Board of the Netafim irrigation company.
A Member of Knesset since April 2006, he served as a member of the Knesset Foreign Affairs & Defense, Ethics, State Control, Labor, Welfare, & Health, and Foreign Workers committees. In September 2007 Ami Ayalon was appointed Minister without Portfolio.
Ayalon is married and the father of three. He has published various articles on national policy, terror, and peace.